Application
Western blot analysis of NIP7 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear staining.Application
Western blot analysis of NIP7 expression in non-transfected: (A) and human NIP7 transfected: (B) 293 whole cell lysates.| Product Name | NIP7 Antibody (X-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against NIP7 of human origin |
| Modification Notes | Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Nip7 is a nucleolar protein involved in ribosome biogenesis, specifically 27S pre-rRNA processing and 60S ribosome subunit assembly in Saccharomyces cerevisiae. NIP7 is a conserved protein among eukaryotes, including human, mouse, rat and pig that is essential for cell growth. In humans, NIP7 interacts with the Shwachman-Bodian-Diamond syndrome (SBDS) protein, which mediates accurate gene expression essential for proper brain, skeletal, and blood cell development. Mutations in the SBDS gene results in an autosomal disorder (SDS) characterized by pleiotropic phenotypes including pancreatic, skeletal and bone marrow deficiencies and predisposition to hematological dysfunctions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of NIP7 of mouse, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against NIP7 of human origin |
|---|---|
| Tissue Specificity | raised against NIP7 of human origin |
| Entrez Gene | 51388 |
|---|
Application
Western blot analysis of NIP7 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear staining.
Application
Western blot analysis of NIP7 expression in non-transfected: (A) and human NIP7 transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of NIP7 of mouse, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
