Application
Western blot analysis of Ezrin phosphorylation in untreated (A) and EGF treated (B) A-431 whole cell lysates.Application
Western blot analysis of phosphorylated Ezrin expression in untreated (A) and EGF-treated (B) A-431 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Product Name | p-Ezrin Antibody (Tyr 354) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 354 of Ezrin of human origin |
| Modification | Phosphoralated |
| Modification Notes | The microvillar core protein Ezrin is a tyrosine-phosphorylated protein found in epidermal growth factor-stimulated A-431 carcinoma cells (3). Ezrin is phosphorylated transiently to a high level on tyrosine residues 146 and 354 (1,2). One of the sites, Tyrosine 146, lies in the amino-terminal region of homology that is common to the Protein 4.1-Talin-Ezrin protein family (1,4). This tyrosine residue and its vicinal amino acids are conserved throughout the family which includes Radixin, Moesin, and the two phosphotyrosine phosphatases, PTP H1 and PTP MEG (1). Another phosphorylation site is Tyrosine 354, which is localized within the alpha-helical domain of Ezrin (1). Cytoplasmic signaling may result in activation of Ezrin in tyrosine phosphorylation and this suggests that Ezrin has qualities that might play a role in modulation of cell shape and adhesion (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Tyr 354 phosphorylated Ezrin of mouse and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 354 of Ezrin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 354 of Ezrin of human origin |
| Entrez Gene | 7430 |
|---|
Application
Western blot analysis of Ezrin phosphorylation in untreated (A) and EGF treated (B) A-431 whole cell lysates.
Application
Western blot analysis of phosphorylated Ezrin expression in untreated (A) and EGF-treated (B) A-431 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Tyr 354 phosphorylated Ezrin of mouse and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
