Application
Western blot analysis of Skp2 p45 expression in SK-N-MC whole cell lysate (A) and A-673 (B) and K-562 (C) nuclear extracts.Application
Western blot analysis of Skp2 p45 expression in A-673 (A), HeLa (B) and K-562 (C) whole cell lysates.| Product Name | Skp2 p45 Antibody (H-435) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-435 representing full length Skp2 p45 of human origin |
| Modification Notes | The critical role that the family of regulatory proteins known as cyclins plays in eukaryotic cell cycle regulation is well established. The best characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of MPF (maturation promoting factor). Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DP-1 and E2F and retino-blastoma protein p110. Two cyclin A-Cdk2 complex binding proteins, Skp1 p19 and Skp2 p45, have been described. Although the Skps (S phase kinase-associated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex. Abolition of Skp2 p45 function by either microinjection of anti-p45 antibodies or addition of antisense oligonucleotides prevents entry into S phase of both normal and transformed cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAChIP |
| recommended for detection of Skp2 p45 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-435 representing full length Skp2 p45 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-435 representing full length Skp2 p45 of human origin |
| Entrez Gene | 6502 |
|---|
Application
Western blot analysis of Skp2 p45 expression in SK-N-MC whole cell lysate (A) and A-673 (B) and K-562 (C) nuclear extracts.
Application
Western blot analysis of Skp2 p45 expression in A-673 (A), HeLa (B) and K-562 (C) whole cell lysates.| Application Notes | recommended for detection of Skp2 p45 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
