Application
Western blot analysis of Amnionless expression in mouse PBL whole cell lysate.| Product Name | Amnionless Antibody (M-228) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 20-248 mapping near the N-terminus of Amnionless of mouse origin |
| Modification Notes | Megaloblastic anemia 1 (MGA1), also referred to as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS), is a hereditary, recessive disorder caused by defects in the AMN gene. Patients suffering from MGA1 have a selective malabsorption of vitamin B12, causing impaired function of thymidine synthase which in turn interrupts DNA synthesis. Amnionless protein, encoded for by the AMN gene, is crucial for vitamin B12 absorption. It modulates a BMP (bone morphogenetic protein) signaling pathway and is therefore important for trunk mesoderm production during development. Amnionless is a membrane protein that interacts with cubulin and is primarily expressed in colon, kidney and small intestine. Shorter isoforms can also be detected in thymus, testis and peripheral blood leukocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Amnionless of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 20-248 mapping near the N-terminus of Amnionless of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 20-248 mapping near the N-terminus of Amnionless of mouse origin |
Application
Western blot analysis of Amnionless expression in mouse PBL whole cell lysate.| Application Notes | recommended for detection of Amnionless of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
