| Product Name | COL12A1 Antibody (H-280) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1861-2140 mapping within an internal region of Collagen Type XII of human origin |
| Modification Notes | Collagen Type XII is a 3,063 amino acid protein encoded by the human gene COL12A1. Collagen Type XII belongs to the fibril-associated collagens with interrupted helices (FACIT) family. Collagen Type XII is known to interact with type I collagen-containing fibrils. The COL1 domain is thought to be associated with the surface of the fibrils, while the COL2 and NC3 domains may be localized in the perifibrillar matrix. Collagen Type XII has 3 identified isoforms (named 1, 2 and 4). Isoform 1 is the long form of the protein, while 2 and 4 are missing sequences found in isoform 1. Collagen Type XII is found in Collagen Type I-containing tissues: both isoform 1 and isoform 2 appear in amnion, chorion, skeletal muscle, small intestine and cell culture of dermal fibroblasts, keratinocytes and endothelial cells. Only isoform 2 is found in lung, placenta, kidney and a squamous cell carcinoma cell line. Isoform 1 is also present in the corneal epithelial Bowman's membrane (BM) and the interfibrillar matrix of the corneal stroma, but it is not detected in the limbal BM. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Collagen Type XII of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1861-2140 mapping within an internal region of Collagen Type XII of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1861-2140 mapping within an internal region of Collagen Type XII of human origin |
| Entrez Gene | 1303 |
|---|
| Application Notes | recommended for detection of Collagen Type XII of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
