Application
Western blot analysis of Gemin8 expression in COLO 320DM whole cell lysate.| Product Name | Gemin8 Antibody (FL-242) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-242 representing full length Gemin8 of human origin |
| Modification Notes | Gemin8 is a 242 amino acid protein encoded by the mouse gene Gemin8. Gemin8, along with Gemins 2-7 and unrip, is a major component of the large multiprotein survival of motor neurons (SMN) complex. The survival of motor neurons (SMN) protein, a product of the disease gene of the common neurodegenerative disease spinal muscular atrophy, is also part of the SMN complex. The SMN complex is a modular composition of proteins with SMN, Gemin8, and Gemin7 in its center. The SMN complex functions as an assembly machine for small nuclear ribonucleoproteins (snRNPs)-the major components of the spliceosome. Gemin8 binds directly to SMN and mediates its interaction with the Gemin6/Gemin7 heterodimer. Importantly, the loss of Gemin6, Gemin7, and Unrip interaction with SMN as a result of Gemin8 knockdown affects snRNP assembly by impairing the SMN complex association with Sm proteins but not with snRNAs. The Gemin6/Gemin7 complex binds to Sm proteins and might help organize Sm proteins for formation of Sm rings on snRNA targets. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Gemin8 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-242 representing full length Gemin8 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-242 representing full length Gemin8 of human origin |
| Entrez Gene | 54960 |
|---|
Application
Western blot analysis of Gemin8 expression in COLO 320DM whole cell lysate.| Application Notes | recommended for detection of Gemin8 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
