Application
Western blot analysis of Spot 14 expression in non-transfected: (A) and human Spot 14 transfected: (B) 293T whole cell lysates.| Product Name | Spot 14 Antibody (H-57) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 90-146 mapping at the C-terminus of Spot 14 of human origin |
| Modification Notes | Spot 14, also known as S14 or THRSP (thyroid hormone responsive protein), is a small acidic protein localizing to the nucleus and can be found in tissues that synthesize triglycerides, such as liver, mammary glands and adipose tissues. Spot 14 is implicated in growth and differentiation, possibly functioning as a transcription regulator for genes encoding proteins that participate in lipogenesis. A variety of lipogenic stimuli can activate the expression of Spot 14, including thyroid hormone, dietary carbohydrate, Insulin and glucose. Its expression can be downregulated by catecholamine and glucagon. In addition, Spot 14 expression is known to oscillate with the circadian clock. Knockdown of Spot 14 leads to impaired lipid synthesis and apoptosis. In most breast cancers, Spot 14 is overexpressed and is believed to augment cell growth and survival. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Spot 14 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 90-146 mapping at the C-terminus of Spot 14 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 90-146 mapping at the C-terminus of Spot 14 of human origin |
| Entrez Gene | 7069 |
|---|
Application
Western blot analysis of Spot 14 expression in non-transfected: (A) and human Spot 14 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Spot 14 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
