Application
Western blot analysis of Myosin Ib expression in non-transfected: (A) and human Myosin Ib transfected: (B) 293T whole cell lysates.| Product Name | Myosin Ib Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1067-1136 mapping at the C-terminus of Myosin Ib of human origin |
| Modification Notes | Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Tro-ponin facilitates interaction between Actin and Myosin by binding to Ca2+. Troponin is made up of at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Myosin is a hexamer of two heavy chains (MHC) and four light chains (MLC) that interacts with Actin to generate the force for diverse cellular movements, including cyto-kinesis, phagocytosis and muscle contraction. Myosin Ib (MYO1B), also designated Myosin I å or MYH-1c, is a motor protein that is involved in cell migration, neurite outgrowth and vesicular transport. In multivesicular endosomes, Myosin Ib has been implicated in protein cargo traffic control. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Myosin Ib of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1067-1136 mapping at the C-terminus of Myosin Ib of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1067-1136 mapping at the C-terminus of Myosin Ib of human origin |
| Entrez Gene | 4430 |
|---|
Application
Western blot analysis of Myosin Ib expression in non-transfected: (A) and human Myosin Ib transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Myosin Ib of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
