Application
Western blot analysis of TS expression in HeLa (A), SH-SY5Y (B), HL-60 (C) and Jurkat (D) whole cell lysates.| Product Name | TS Antibody (H-265) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 49-313 mapping at the C-terminus of TS of human origin |
| Modification Notes | Thymidylate synthase (TS), also designated TYMS, TMS, TSase and HsT422, uses 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor in the synthesis of 2'-deoxythymidine-5'-monophosphate (dTMP), an essential precursor for DNA biosynthesis. TS is an RNA-binding protein that can interact with its own mRNA. The TS/mRNA ribonucleoprotein complex can also associate with a number of other cellular mRNAs, including those corresponding to the p53 tumor suppressor gene and the Myc family of transcription factors. Inhibition of DNA replication and cell death resulting from thymidine depletion occurs when TS enzyme activity is inhibited with substrate or cofactor analogs, making the TS enzyme an important target for chemotherapy. Cancer cells are sensitive to thymidine depletion, as they multiply rapidly. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 49-313 mapping at the C-terminus of TS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 49-313 mapping at the C-terminus of TS of human origin |
Application
Western blot analysis of TS expression in HeLa (A), SH-SY5Y (B), HL-60 (C) and Jurkat (D) whole cell lysates.| Application Notes | recommended for detection of TS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
