Application
Immunoperox-idase staining of formalin fixed, paraffin-embedded human rectum tissue showing nuclear staining of glandular cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Positive cofactor 4 expression in non-transfected: (A) and human Positive cofactor 4 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Positive cofactor 4 expression in non-transfected 293: (A), human Positive cofactor 4 transfected 293: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Positive cofactor 4 expression in non-transfected: (A) and human Positive cofactor 4 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Positive cofactor 4 expression in Jurkat nuclear extract.| Product Name | Positive cofactor 4 Antibody (H-114) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 14-127 mapping at the C-terminus of Positive cofactor 4 of human origin |
| Modification Notes | In eukaryotic cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase. RNA polymerase II (RNAPII) holoenzyme contains numerous proteins that largely consist of RNA processing factors, RNA helicase, general transcription factors and SRB co-activating complexes. RNAPII mediated basal- and gene-specific transcriptional activation requires the association of various cofactors that includes PC4 (human Positive cofactor 4). Positive cofactor 4 interacts with the activation domain of transcription factor IIA (TFIIA) and TATA-binding protein (TBP)-associated factors (TAFs) to directly bind to double stranded DNA. Positive cofactor 4 induces both activation and repression of RNAPII basal transcription, depending on the presence or absence of these transcription factors and holoenzyme components. Addition-ally, Positive cofactor 4 is phosphorylated by TFIID and TFIIH, which releases Positive cofactor 4 from the DNA promoter region and thereby inhibits the assembly of Positive cofactor 4 into the transcriptional promoting complex and blocks transcription. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Positive cofactor 4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 14-127 mapping at the C-terminus of Positive cofactor 4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 14-127 mapping at the C-terminus of Positive cofactor 4 of human origin |
| Entrez Gene | 10923 |
|---|
Application
Immunoperox-idase staining of formalin fixed, paraffin-embedded human rectum tissue showing nuclear staining of glandular cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Positive cofactor 4 expression in non-transfected: (A) and human Positive cofactor 4 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Positive cofactor 4 expression in non-transfected 293: (A), human Positive cofactor 4 transfected 293: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Positive cofactor 4 expression in non-transfected: (A) and human Positive cofactor 4 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Positive cofactor 4 expression in Jurkat nuclear extract.| Application Notes | recommended for detection of Positive cofactor 4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
