Application
Western blot analysis of NPC2 expression in Hep G2 whole cell lysate.Application
Western blot analysis of NPC2 expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of NPC2 expression in non-transfected: (A) and mouse NPC2 transfected: (B) 293T whole cell lysates.| Product Name | NPC2 Antibody (H-125) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-145 mapping within an internal region of NPC2 of human origin |
| Modification Notes | Niemann-Pick disease, type C2 (NPC2), also known as epididymal secretory protein, is a secreted protein mapping against gene 14q24.3 (1,2). NPC2 regulates the lipid composition of sperm membranes during maturation in the epididymis(1,2). Mutations in the NPC2 gene may cause Nieman-Pick type C2 disease and frontal lobe atrophy (1,2,3). Nieman-Pick type C2 is a fatal hereditary disease characterized by defective lysosome release of cholesterol (3). The disease is caused by HE1 deficiency, a lysosmal protein proven to be undetectable in fibroblasts from NPC2 patients (3). This differentiates NPC2 from NPC1, as NPC1 has HE1 protein present (3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NPC2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-145 mapping within an internal region of NPC2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-145 mapping within an internal region of NPC2 of human origin |
| Entrez Gene | 10577 |
|---|
Application
Western blot analysis of NPC2 expression in Hep G2 whole cell lysate.
Application
Western blot analysis of NPC2 expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of NPC2 expression in non-transfected: (A) and mouse NPC2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of NPC2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
