Application
Western blot analysis of human recombinant Nanog fusion protein.| Product Name | Nanog Antibody (H-155) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 151-305 mapping at the C-terminus of Nanog of human origin |
| Modification Notes | Nanog (from “Tir Na Nog,” the mythologic Celtic land of the ever young) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression have been detected by Northern analysis in the undifferentiated NTERA-2 cl.D1 embryonal carcinoma cell line. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Nanog of human origin by WB, IP, IF and ELISA also recommended for detection of NanogP1 and NanogP8: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 151-305 mapping at the C-terminus of Nanog of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 151-305 mapping at the C-terminus of Nanog of human origin |
| Entrez Gene | 79923 |
|---|
Application
Western blot analysis of human recombinant Nanog fusion protein.| Application Notes | recommended for detection of Nanog of human origin by WB, IP, IF and ELISA also recommended for detection of NanogP1 and NanogP8: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
