Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of GABP-α expression in non-transfected: (A) and human GABP-α transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GABP-α expression in HeLa (A), MCF7 (B), SK-BR-3 (C) and SW480 (D) nuclear extracts.| Product Name | GABP-α Antibody (H-180) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-180 mapping at the N-terminus of GABP-α of human origin |
| Modification Notes | The transcription factor GA-binding protein (GABP) is composed of two subunits, the Ets-related GABP-å and a GABP-å-associated subunit, GABP∫. GABPå binds to a specific DNA sequence and GABP∫ exists as b1 and b2 splice variants that differ in their C-termini. In primary neuronal cultures, GABP∫ is expressed in both the cytoplasm and the nucleus, whereas GABPå is expressed mainly in the nucleus. GABP is constitutively expressed as either a GABPå∫ heterodimer or a GABPåb heterotetramer, both of which can modify GABP-dependent transcription in vitro and in vivo. The GABPå∫ tetrameric complex performs many different functions, such as stimulating transcription of the adenovirus E4 gene, differentially activating BRCA1 expression in human breast cell lines, potentiating Tat-mediated activation of long terminal repeat promoter transcription and viral replication in certain cell types, acting as a coordinator of mitochrondrial and nuclear transcription for cytochrome oxidase in neurons and assisting in the regulation of rpL32 gene transcription. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of GABP-α of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-180 mapping at the N-terminus of GABP-&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-180 mapping at the N-terminus of GABP-α of human origin |
| Entrez Gene | 2551 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of GABP-α expression in non-transfected: (A) and human GABP-α transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GABP-α expression in HeLa (A), MCF7 (B), SK-BR-3 (C) and SW480 (D) nuclear extracts.| Application Notes | recommended for detection of GABP-α of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
