Application
Western blot analysis of mHMGCS expression in non-transfected: (A) and mouse mHMGCS transfected: (B) 293T whole cell lysates.Application
Western blot analysis of mHMGCS expression in non-transfected: (A) and mouse mHMGCS transfected: (B) 293T whole cell lysates.Application
Western blot analysis of mHMGCS expression in mouse placenta tissue extract.| Product Name | mHMGCS Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 419-488 mapping near the C-terminus of mHMGCS of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | HMG-CoA Synthase exists as both a mitochondrial (mHMGCS) and cytoplasmic (cHMGCS) enzyme that condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA. The HMG-CoA produced by cHMGCS is transformed into mevalonate by HMG-CoA reductase, which starts isoprenoid biosynthesis. End products of the isoprenoid pathway include cholesterol, ubiquinone, dolichol, isopentenyl adenosine and farnesyl groups. mHMGCS, together with HMG-CoA Lyase, is responsible for ketone body biosynthesis. mHMGCS is expressed in liver and kidney. Fasting, cAMP and fatty acids increase the level of transcription of mHMGCS, while feeding and insulin repress it. A regulatory element within the mHMGCS promoter confers transcriptional regulation by PPAR, RXR, COUP-TF and HNF-4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 419-488 mapping near the C-terminus of mHMGCS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 419-488 mapping near the C-terminus of mHMGCS of human origin |
| Entrez Gene | 3158 |
|---|
Application
Western blot analysis of mHMGCS expression in non-transfected: (A) and mouse mHMGCS transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of mHMGCS expression in non-transfected: (A) and mouse mHMGCS transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of mHMGCS expression in mouse placenta tissue extract.| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
