Application
Western blot analysis of yeast recombinant Aut7 fusion protein.| Product Name | Aut7 Antibody (y-117) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-117 representing full length Aut7 of Saccharomyces cerevisiae origin |
| Modification Notes | Autophagy, an intracellular degradation system, is a process in which cytoplasmic components are enclosed in autophagosomes and delivered to lysosomes (1-5). Autophagy in yeast requires a protein conjugation system consisting of Apg12 covalently bound at the carboxy terminal glycine to lysine 149 of Apg5 (3). Apg7 is a protein-activating enzyme that is similar to E1 family ubiquitin-activating enzymes (1-3). Apg7 is required for the Apg12-Apg5 conjugation to occur and is essential for normal cytoplasm-to-vacuole targeting, autophagy and peroxisome degradation pathways (1). Aut7 expression is induced by nitrogen starvation and is required for cytoplasm to vacuole targeting (Cvt) and autophagy (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Aut7 of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-117 representing full length Aut7 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-117 representing full length Aut7 of Saccharomyces cerevisiae origin |
Application
Western blot analysis of yeast recombinant Aut7 fusion protein.| Application Notes | recommended for detection of Aut7 of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
