Application
Western blot analysis of EHHADH expression in non-transfected 293T: (A), mouse EHHADH transfected 293T: (B), Hep G2 (C) and Caki-1 (D) whole cell lysates and mouse liver tissue extract (E).Application
Western blot analysis of EHHADH expression in non-transfected: (A) and mouse EHHADH transfected: (B) 293T whole cell lysates.Application
Western blot analysis of EHHADH expression in mouse kidney tissue extract.| Product Name | EHHADH Antibody (K-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of EHHADH of human origin |
| Modification Notes | Peroxisomes play an important role in the oxidation of fatty acids via ∫-oxidation, which is carried out by two distinct pathways; the L-hydroxy-specific classical ∫-oxidation for very long straight-chain fatty acids and the D-hydroxy-specific ∫-oxidation for branched-chain fatty acids. A defect in either pathway can result in elevated serum levels of fatty-acids, leading to severe mental retardation and early death. As an L-hydroxy-specific enzyme, EHHADH (enoyl-CoA-hydratase:3-hydroxyacyl-CoA dehydrogenase), also known as Peroxisomal L-bifunctional enzyme, is a 723 amino acid protein has an essential tripeptide sequence on its carboxyl-terminus that is required for peroxisomal transport. EHHADH-null mice only exhibit a blunted peroxisome proliferative response when challenged with a peroxisome proliferator. Since there were no observed changes in lipid metabolism, this evidence suggests that enoyl-CoAs were diverted to the D-hydroxy-specific ∫-oxidation system for metabolism. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of EHHADH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of EHHADH of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of EHHADH of human origin |
| Entrez Gene | 1962 |
|---|
Application
Western blot analysis of EHHADH expression in non-transfected 293T: (A), mouse EHHADH transfected 293T: (B), Hep G2 (C) and Caki-1 (D) whole cell lysates and mouse liver tissue extract (E).
Application
Western blot analysis of EHHADH expression in non-transfected: (A) and mouse EHHADH transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of EHHADH expression in mouse kidney tissue extract.| Application Notes | recommended for detection of EHHADH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
