Application
Western blot analysis of TMED4 expression in non-transfected: (A) and human TMED4 transfected: (B) 293T whole cell lysates and mouse liver tissue extract (C).Application
Western blot analysis of TMED4 expression in human liver tissue extract.Application
Western blot analysis of TMED4 expression in EOC 20 whole cell lysate.| Product Name | TMED4 Antibody (A-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of TMED4 of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | TMED4 (Transmembrane emp24 domain-containing protein 4), also known as ERS25 (Endoplasmic reticulum stress-response protein 25) and Putative NF-kappa-B-activating protein 156, is a 225 amino acid protein that is a member of the EMP24/GP25L family. Like most members of this family, TMED4 is a single-pass type I membrane protein containing one GOLD domain. The GOLD (golgi dynamics) domain is a region of about 90 to 150 amino acids that mediates protein-protein interactions. The GOLD domain interacts with lipid, sterol or fatty acid-domains as well as with the RUN domain, which interacts with cytoskeletal filaments, of membrane proteins. Localized to the endoplasmic reticulum, TMED4 is induced by ER-specific stress, heat shock and oxidative stress. Knockdown of TMED4 mRNA results in a significant reduction in apoptosis as well as a reduction in reactive oxidative species. There are three isoforms of TMED4 that are produced as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of TMED4 of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of TMED4 of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 222068 |
|---|
Application
Western blot analysis of TMED4 expression in non-transfected: (A) and human TMED4 transfected: (B) 293T whole cell lysates and mouse liver tissue extract (C).
Application
Western blot analysis of TMED4 expression in human liver tissue extract.
Application
Western blot analysis of TMED4 expression in EOC 20 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
