Application
Western blot analysis of IDE expression in MES-SA/Dx5 (A), Hep G2 (B) and A-375 (C) whole cell lysates.Application
Western blot analysis of IDE expression in A-375 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.| Product Name | IDE Antibody (A-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of IDE of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Insulin degrading enzyme (IDE), initiates the cleavage of insulin, resulting in insulin response and resistance. However, IDE also degrades a variety of bioactive peptides, including amyloid-∫ peptides, implicating IDE in certain age-related changes seen in Alzheimer’s disease. Studies show that when the expression of the IDE gene (chromosome 10q23.3) is altered, changes occur not only in glucose homeostasis, but also in the levels of brain Abeta40 and Abeta42 peptides. An IDE inhibitor, bacitracin, inhibits degradation of both insulin and amylin, indicating that both are degraded through a common proteolytic pathway. Variations in the rate of proteolysis suggest that the function of IDE exhibits conformational dependence, which may lead to possible therapeutic interventions for diabetes, AD, and other diseases associated with IDE substrate proteolysis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of IDE of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of IDE of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 3416 |
|---|
Application
Western blot analysis of IDE expression in MES-SA/Dx5 (A), Hep G2 (B) and A-375 (C) whole cell lysates.
Application
Western blot analysis of IDE expression in A-375 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
