Application
Western blot analysis of SLA/LP expression in non-transfected: (A) and mouse SLA/LP transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SLA/LP expression in Jurkat whole cell lysate.Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining.| Product Name | SLA/LP Antibody (W-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against SLA/LP of human origin |
| Modification Notes | The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Aminoacyl-tRNA synthetases function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets.SLA/LP (Soluble liver antigen/Liver-pancreas antigen), also known as SEPSECS (Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase) or SLA-p35, is a 501 amino acid cytoplasmic protein that belongs to a diverse family of pyridoxal phosphate-dependent enzymes. Expressed predominantly in liver, lung, kidney and pancreas, SLA/LP plays a role in aminoacyl-tRNA synthesis and, more specifically, selenoprotein biosynthesis. Using PLP as a cofactor, SLA/LP specifically converts O-phosphoseryl-tRNA(Sec) to Sec-tRNA(Sec) by exchanging the phosphate group for a selenol moiety. Due to alternative splicing events, two SLA/LP isoforms exist. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SLA/LP of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against SLA/LP of human origin |
|---|---|
| Tissue Specificity | raised against SLA/LP of human origin |
| Entrez Gene | 51091 |
|---|
Application
Western blot analysis of SLA/LP expression in non-transfected: (A) and mouse SLA/LP transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SLA/LP expression in Jurkat whole cell lysate.
Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining.| Application Notes | recommended for detection of SLA/LP of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
