Application
Western blot analysis of POR1 expression in non-transfected 293T: (A), human POR1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of POR1 expression in HeLa whole cell lysate.Application
Western blot analysis of POR1 expression in non-transfected: (A) and mouse POR1 transfected: (B) 293T whole cell lysates.| Product Name | POR1 Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-100 mapping at the N-terminus of POR1 of human origin |
| Modification Notes | POR1 (also designated arfaptin 2) was first isolated as a Rac 1 binding protein necessary for Rac-mediated Actin polymerization and the subsequent formation of membrane ruffles and lamellipodia. POR1 has also been shown to interact with the ADP ribosylation factor ARF6, a GTPase that associates with the plasma membrane and intracellular endosome vesicles, in a GTP dependent manner. The association of POR1 with ARF6 stimulates induction of Actin polymerization. POR1 appears to play a regulatory role through mul-tiple signaling pathways in the reorganization of the cytoskeletal structure. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of POR1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-100 mapping at the N-terminus of POR1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-100 mapping at the N-terminus of POR1 of human origin |
| Entrez Gene | 23647 |
|---|
Application
Western blot analysis of POR1 expression in non-transfected 293T: (A), human POR1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of POR1 expression in HeLa whole cell lysate.
Application
Western blot analysis of POR1 expression in non-transfected: (A) and mouse POR1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of POR1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
