Application
Western blot analysis of PRL-R expression in 293T (A) and A-431 (B) whole cell lysates and mouse liver (C), mouse pancreas (D), mouse kidney (E) and mouse small intestine (F) tissue extracts.Application
Western blot analysis of mouse recombinant PRL-R.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.| Product Name | PRL-R Antibody (M-170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 91-260 mapping within an extracellular domain of PRL-R of mouse origin |
| Modification Notes | The anterior pituitary secretes a variety of hormones that are involved in cell growth, differentiation and development. Prolactin, a 226 amino acid protein, plays a role in multiple processes, including cell growth, reproduction and immune function. Full length prolactin, as well as an alternative splice product lacking the third exon, are secreted by endothelial cells involved in angiogenesis. In addition to its role in mammary development and lactation, prolactin is known to play a role in the development of mammary cancer, acting as both a mitogen and a differentiating agent. Prolactin has also been shown to enhance the proliferation of B cell hybridomas, leading to an overall increase in antibody production. Prolactin reverses the antiproliferative effects of the immunosuppressive cytokine TGF-beta. Prolactin is also associated with a variety of autoimmune diseases, including arthritis and type 1 diabetes. The receptor for Prolactin (PRL-R) belongs to the cytokine receptor superfamily. PRL-R is activated by ligand-induced homodimerization and subsequent cell signaling through the JAK/Stat pathway. The gene encoding human PRL-R maps to chromosome 5p13.2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PRL-R isoforms 1-3 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 91-260 mapping within an extracellular domain of PRL-R of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 91-260 mapping within an extracellular domain of PRL-R of mouse origin |
| Entrez Gene | 5618 |
|---|
Application
Western blot analysis of PRL-R expression in 293T (A) and A-431 (B) whole cell lysates and mouse liver (C), mouse pancreas (D), mouse kidney (E) and mouse small intestine (F) tissue extracts.
Application
Western blot analysis of mouse recombinant PRL-R.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.| Application Notes | recommended for detection of PRL-R isoforms 1-3 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
