Application
Western blot analysis of prefoldin 5 expression in NIH/3T3 (A) and HeLa (B) nuclear extracts.Application
Western blot analysis of prefoldin 5 expression in non-transfected: (A) and mouse prefoldin 5 transfected: (B) 293T whole cell lysates.| Product Name | prefoldin 5 Antibody (FL-154) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-154 representing full length prefoldin 5 of human origin |
| Modification Notes | Molecular chaperones are proteins that assist in the correct folding of other proteins in the crowded molecular environment that exists in living cells (1). Within this class of proteins, a key role is played by chaperonins, multisubunit toroidal (i.e. doughnut-shaped) assemblies that undergo major ATP-dependent conformational changes as part of the mechanism of facilitated folding. Prefoldin is a heterohexameric chaperone protein which has the ability to capture unfolded Actin. Six prefoldin polypeptides, prefoldin 1-6, have been identified. Prefoldin 1 is a 122 amino acid protein that binds specifically to cytosolic chaperonin (c-cpn) and transfers target proteins to it. Prefoldin 3 (VBP1 or VHL binding protein-1) forms complexes with VHL and is translocated from perinuclear granules to the nucleus or cytoplasm. Prefoldin 4 is a possible transcription factor. Prefoldin 5 (c-Myc-binding protein Mm-1, Myc modulator 1 or MM-1) is a c-Myc binding protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of prefoldin 5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-154 representing full length prefoldin 5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-154 representing full length prefoldin 5 of human origin |
| Entrez Gene | 5204 |
|---|
Application
Western blot analysis of prefoldin 5 expression in NIH/3T3 (A) and HeLa (B) nuclear extracts.
Application
Western blot analysis of prefoldin 5 expression in non-transfected: (A) and mouse prefoldin 5 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of prefoldin 5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
