Application
Antibodies tested include p-NFATc4 (Ser 168/170): (A,B) and NFATc4 (C-20): (C,D).Application
Western blot analysis of NFATc4 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) Jurkat nuclear extracts.Application
Antibodies tested include p-NFATc4 (Ser 168/170): (A, B) and NFATc4 (H-74): (C, D).Application
Western blot analysis of NFATc4 phosphorylation in non-transfected: (A) and mouse NFATc4 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NFATc4 phosphorylation in non-transfected: (A) and human NFATc4 transfected: (B) 293T whole cell lysates.| Product Name | p-NFATc4 Antibody (Ser 168/170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 168 and Ser 170 dually phosphorylated NFATc4 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine, bovine, porcine and avian |
| Modification | Phosphoralated |
| Modification Notes | NFATc4 (nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4) is a member of the nuclear factors of activated T cells DNA-binding transcription complex that influences cytokine gene expression, cardiac hypertrophy, and adipocyte differentiation. This complex consists of at least two components: a cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Other members of this family participate in the formation of this complex. NFATc4 plays a role in the inducible expression of cytokine genes in T cells, including the induction of IL-2 and IL-4. p38 MAP kinase phosphorylates multiple residues, including Serine-168 and Serine-170, in the NFAT homology domain of NFATc4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| blocking peptide, sc-32630 P: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 168 and Ser 170 dually phosphorylated NFATc4 of mouse rat and human origin by WB IP IF and ELISA also reactive with additional species including canine bovine porcine and avian |
|---|---|
| Tissue Specificity | recommended for detection of Ser 168 and Ser 170 dually phosphorylated NFATc4 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine, bovine, porcine and avian |
| Entrez Gene | 4776 |
|---|
Application
Antibodies tested include p-NFATc4 (Ser 168/170): (A,B) and NFATc4 (C-20): (C,D).
Application
Western blot analysis of NFATc4 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) Jurkat nuclear extracts.
Application
Antibodies tested include p-NFATc4 (Ser 168/170): (A, B) and NFATc4 (H-74): (C, D).
Application
Western blot analysis of NFATc4 phosphorylation in non-transfected: (A) and mouse NFATc4 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NFATc4 phosphorylation in non-transfected: (A) and human NFATc4 transfected: (B) 293T whole cell lysates.| Application Notes | blocking peptide, sc-32630 P: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
