Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human stomach tissue showing nuclear and cytoplasmic localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic localization.| Product Name | DAZ4 Antibody (J-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of DAZ4 of human and canine origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Spermatogenesis is the process by which male spermatogonia develop into mature spermatozoa. DAZ (deleted in azoospermia) are RNA-binding proteins that play an essential role in spermatogenesis. DAZ proteins influence the first stages of spermatogenesis and the maintenance of germ cell populations. DAZ proteins (DAZ1, DAZ2, DAZ3, DAZ4 and DAZ5) are encoded by separate genes on chromosome Y, each of which contain an AZFc domain in their coding region. DAZ proteins localize to the nucleus of spermatogonia, but relocate to the cytoplasm during meiosis. DAZ proteins contain an RRM (RNA recognition motif) domain that may regulate mRNA translation by binding to the 3’UTR. Deletions in the genes encoding DAZ proteins may cause azoospermia or oligospermia which can lead to male infertility. DAZ4 (deleted in azoospermia 4), also known as pDP1680 or pDP1681, is a 579 amino acid testis specific protein that contains nine DAZ-like domains and two RNA recognition motifs (RRM). DAZ4 exists as two alternatively spliced isoforms. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of DAZ4 of human and canine origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of DAZ4 of human and canine origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 57135 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human stomach tissue showing nuclear and cytoplasmic localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
