Application
Western blot analysis of SPATA12 expression in Jurkat whole cell lysate.| Product Name | SPATA12 Antibody (K-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of SPATA12 of human origin by WB, IP and ELISA |
| Modification Notes | SPATA12 (Spermatogenesis-associated protein 12), also known as SRG5 (Spermatogenesis-related protein 5), is a 190 amino acid protein that may be involved in the regulation of spermatogenesis. SPATA12 is expressed in normal adult testis, but is absent in fetal testis, implying that it may be involved in the development of testicular maturation. Specifically, SPATA12 is expressed in spermatocytes, spermatids and spermatozoa, with no expression in Leydig and Sertoli cells. Studies with HeLa and GC-1 germ cells indicate that expression of the SPATA12 gene may delay the progression of G1 to S in the cell cycle, therefore it is thought that SPATA12 maintains the cell in a differentiated state and suppresses cell proliferation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SPATA12 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SPATA12 of human origin by WB, IP and ELISA |
| Entrez Gene | 353324 |
|---|
Application
Western blot analysis of SPATA12 expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
