Application
Western blot analysis of RNF10 expression in non-transfected: (A) and human RNF10 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RNF10 expression in non-transfected: (A) and human RNF10 transfected: (B) 293 whole cell lysates.| Product Name | RNF10 Antibody (V-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of RNF10 of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF10 (ring finger protein 10), also known as RIE2, is an 811 amino acid protein that localizes to the cytoplasm and contains one RING-type zinc finger. Existing as multiple alternatively spliced isoforms, RNF10 interacts with MOX-2 and is thought to regulate its transcription in schwann cells, possibly playing a role in myelin formation. The gene encoding RNF10 maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and Trisomy 12p, which causes facial developmental defects and seizure disorders. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of RNF10 of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of RNF10 of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 9921 |
|---|
Application
Western blot analysis of RNF10 expression in non-transfected: (A) and human RNF10 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RNF10 expression in non-transfected: (A) and human RNF10 transfected: (B) 293 whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
