Application
Western blot analysis of BPI expression in non-transfected: (A) and human BPI transfected: (B) 293T whole cell lysates.Application
Western blot analysis of BPI expression in HL-60 (A) and CCRF-CEM (B) whole cell lysates.| Product Name | BPI Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 321-450 mapping near the C-terminus of BPI of human origin |
| Modification Notes | The bactericidal permeability increasing protein (BPI) is an antibacterial and endotoxin-neutralizing molecule that is abundant in the granules of polymorphonuclear leukocytes (neutrophil granules). The 31.5-kb-long human BPI gene maps to chromosome 20q11.23-q12, contains 15 exons, and encodes a 456 amino acid protein. Epithelial cells which line mucosal surfaces are the first line of defense against bacterial invasion and infection. BPI localizes to the cell surface of epithelial cells and blocks endotoxin-mediated signaling, thereby protecting mucosal surfaces against gram-negative bacteria and their endotoxin. BPI, lipopolysaccharide binding protein (LBP), phospholipid transfer protein (PLTP), and cholesteryl ester transfer protein (CETP) constitutes a family of functionally related proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of BPI of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 321-450 mapping near the C-terminus of BPI of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 321-450 mapping near the C-terminus of BPI of human origin |
| Entrez Gene | 671 |
|---|
Application
Western blot analysis of BPI expression in non-transfected: (A) and human BPI transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of BPI expression in HL-60 (A) and CCRF-CEM (B) whole cell lysates.| Application Notes | recommended for detection of BPI of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
