Application
Western blot analysis of Neu1 expression in non-transfected: (A) and human Neu1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Neu1 expression in Hep G2 whole cell lysate.WB
B Western blot detection of neuraminidase in NAV, PMV, TSV and platelets by NEU-1 polyclonal antibody.| Product Name | Neu1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | NEU1 encodes the lysosomal enzyme neuraminidase, Neu1, which cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids (1,2). In the lysosome Neu1 belongs to a heterotrimeric complex containing beta-galactosidase and cathepsin A (also referred to as 'protective protein') (1). In humans, primary or secondary deficiency of this enzyme leads to two clinically similar neurodegenerative lysosomal storage disorders: sialidosis and galactosialidosis (GS) (1-3). Sialidosis symptoms range from eye abnormalities and neurological disturbances to skeletal malformations, mental retardation and early death (4). Neu1 is expressed in the pancreas, muscle, kidney, placenta, heart, lung and liver (5). The human Neu1 gene maps to chromosome 6p21.33 and encodes a lysosomal protein localized on the inner side of the plasma membrane and in intracellular vesicles (5). Neu1 is also known as alpha-N-acetyl-neuraminidase and Acetylneuraminyl hydrolase (5-6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| recommended for detection of Neu1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 116-415 mapping at the C-terminus of Neu1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 116-415 mapping at the C-terminus of Neu1 of human origin |
| Entrez Gene | 4758 |
|---|
Application
Western blot analysis of Neu1 expression in non-transfected: (A) and human Neu1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Neu1 expression in Hep G2 whole cell lysate.
WB
B Western blot detection of neuraminidase in NAV, PMV, TSV and platelets by NEU-1 polyclonal antibody.| Application Notes | recommended for detection of Neu1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
