Application
Western blot analysis of AIP2 expression in non-transfected 293T: (A), human AIP2 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of AIP2 expression in non-transfected: (A) and human AIP2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of AIP2 expression in non-transfected: (A) and human AIP2 transfected: (B) 293 whole cell lysates.| Product Name | AIP2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of AIP2 of human origin |
| Modification Notes | Atrophin interacting proteins (AIP)s bind to atrophin-1 in the vicinity of the polyglutamine tract. The WW domain consists of 35-40 amino acids and is characterized by four well conserved aromatic residues, two of which are tryptophan. All five AIPs contain multiple WW domains and can be divided into two distinct classes. AIP1 and AIP3 (WWP3) are MAGUK-like multidomain proteins containing a guanylate kinase-like region, two WW domains and multiple PDZ domains. AIP2 (WWP2), AIP4 (itchy) and AIP5 (WWP1) are highly homologous, each having four WW domains and a HECT domain characteristic of ubiquitin ligases. These interactors are similar to isolated Huntingtin-interacting proteins, suggesting commonality of function between two families of proteins responsible for similar diseases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AIP2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of AIP2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of AIP2 of human origin |
| Entrez Gene | 11060 |
|---|
Application
Western blot analysis of AIP2 expression in non-transfected 293T: (A), human AIP2 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of AIP2 expression in non-transfected: (A) and human AIP2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of AIP2 expression in non-transfected: (A) and human AIP2 transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of AIP2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
