Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of HXK I expression in non-transfected: (A) and human HXK I transfected: (B) 293T whole cell lysates.Application
Western blot analysis of human recombinant HXK I fusion protein.| Product Name | HXK I Antibody (H-95) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 316-410 mapping within an internal region of HXK I of human origin |
| Modification Notes | The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HXK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines. HXK II is involved in the uptake and utilization of glucose by adipose and skeletal tissues. Of the hexokinases, HXK III has the highest affinity for glucose. Glucokinase is expressed in pancreatic beta cells where it functions as a glucose sensor, determining the “set point” for insulin secretion. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of HXK I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 316-410 mapping within an internal region of HXK I of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 316-410 mapping within an internal region of HXK I of human origin |
| Entrez Gene | 3098 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of HXK I expression in non-transfected: (A) and human HXK I transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of human recombinant HXK I fusion protein.| Application Notes | recommended for detection of HXK I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
