Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing membrane staining of glandular cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of SR-5A expression in IMR-32 whole cell lysate.| Product Name | SR-5A Antibody (H-57) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-57 mapping at the N-terminus of SR-5A of human origin |
| Modification Notes | SR-5B, also designated 5-hydroxytryptamine (serotonin) receptor 5B (5-HT5B) and G protein-coupled receptor 134 (GPR134) is a receptor for the monoamine ligand serotonin (5-hydroxytryptamine, 5-HT). Serotonin is a neurotransmitter derived from serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin actions are mediated by receptors that influence the biochemistry of depression, anxiety, sexuality and appetite. Rat SR-5B is present in serotonergic neurons in dorsal raphe (DR) and central superior nucleus (CS, median raphe nucleus). DR cell bodies showing SR-5B mRNA expression are abundant in the medial portions of the nucleus. CS coexpression of SR-5B receptor mRNA with serotonin transporter mRNA is high in the intermediate portions of the nucleus. Serotonin receptors include SR-1–7 (5HT1–7). Subtypes within the SR-1 group include SR-1A, -1B, -1D, -1E and -1F. Subtypes within the SR-2 group include SR-2A, -2B and -2C. Subtypes within the SR-5 group include SR-5A and -5B. SR receptors can couple to G proteins that act on either adenylate cyclase or phospholipase C (PLC). The SR-3 class of receptors are ion channels.32234 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SR-5A of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-57 mapping at the N-terminus of SR-5A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-57 mapping at the N-terminus of SR-5A of human origin |
| Entrez Gene | 3361 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing membrane staining of glandular cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of SR-5A expression in IMR-32 whole cell lysate.| Application Notes | recommended for detection of SR-5A of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
