Application
Western blot analysis of Su(fu) expression in non-transfected 293T: (A), human Su(fu) transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Su(fu) expression in C32 (A) and SK-N-SH (B) whole cell lysates.| Product Name | Su(fu) Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 185-484 mapping at the C-terminus of suppressor of fused of human origin |
| Modification Notes | Su(fu) (for Suppressor-of-Fused) is a key negative regulator in the vertebrate Hedgehog signaling pathway. Su(fu) interacts with genes encoding proteins in this signal transduction pathway. In Drosophila, Intracellular transduction of the Hedgehog pathway involves the release of a large complex containing Su(fu). Su(fu) inhibits the activity of the transcription factor Gli1 and interacts with Gli2, Gli3 and the serine/threonine kinase Fused. Su(fu) is widely expressed in adult and embryonic tissues with higher expression in tissues patterned by hegdehog signaling. The Su(fu) gene locus maps to a region that is deleted in glioblastomas, prostate cancer, malignant melanoma and endometrial cancer. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Su(fu) 54 kDa form (484 amino acid splice variant) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 185-484 mapping at the C-terminus of suppressor of fused of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 185-484 mapping at the C-terminus of suppressor of fused of human origin |
| Entrez Gene | 51684 |
|---|
Application
Western blot analysis of Su(fu) expression in non-transfected 293T: (A), human Su(fu) transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Su(fu) expression in C32 (A) and SK-N-SH (B) whole cell lysates.| Application Notes | recommended for detection of Su(fu) 54 kDa form (484 amino acid splice variant) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
