Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate tissue showing membrane staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.Application
Western blot analysis of spectrin β III expression in SK-N-SH whole cell lysate.| Product Name | spectrin β III Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 2311-2380 mapping near the C-terminus of spectrin β III of human origin |
| Modification Notes | Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Spectrin å and spectrin ∫ are present in erythrocytes, whereas spectrin å II (also designated fodrin å) and spectrin ∫ I (also designated fodrin ∫) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin ∫ III is highly homologous to both spectrin ∫ I and spectrin ∫ II. Western blot analysis shows that spectrin ∫ III migrates at a higher molecular mass than predicted in the kidney. Spectrin ∫ III is highly expressed in brain, kidney, pancreas, and liver, and at lower levels in lung and placenta. Specifically, spectrin ∫ III constitutes a major component of the Golgi and vesicular membrane skeletons. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of spectrin β III of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 2311-2380 mapping near the C-terminus of spectrin &beta III of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 2311-2380 mapping near the C-terminus of spectrin β III of human origin |
| Entrez Gene | 6712 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate tissue showing membrane staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.
Application
Western blot analysis of spectrin β III expression in SK-N-SH whole cell lysate.| Application Notes | recommended for detection of spectrin β III of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
