Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic and membrane staining of Purkinje cells and cytoplasmic staining of cells in granular layer and cells in molecular layer.Application
Western blot analysis of Stim1 expression in non-transfected 293T: (A), human Stim1 transfected 293T: (B) and A-10 (C) whole cell lysates.| Product Name | Stim1 Antibody (H-180) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 441-620 mapping near the C-terminus of Stim1 of human origin |
| Modification Notes | Ca2+ influx is essential for a variety of cellular functions including, secretion and transcription. Stromal interaction molecule 1 (Stim1) is a ubiquitously expressed cell surface transmembrane glycoprotein that plays a role in medi-ating Ca2+ influx following the depletion of intracellular Ca2+ stores. Stim1 functions in the endoplasmic reticulum (ER) where it acts as a Ca2+ sensor via its EF-hand domain which causes large conformational changes. When Ca2+ levels drop, Stim1 translocates from the ER to the plasma membrane, where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit, TMEM142A/Orai1. Stim2 is a potent inhibitor of Stim1-mediated store-operated calcium (SOC) entry. Stim1 is implicated in tumor growth suppression and stromal-hematopoietic cell interactions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Stim1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 441-620 mapping near the C-terminus of Stim1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 441-620 mapping near the C-terminus of Stim1 of human origin |
| Entrez Gene | 6786 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic and membrane staining of Purkinje cells and cytoplasmic staining of cells in granular layer and cells in molecular layer.
Application
Western blot analysis of Stim1 expression in non-transfected 293T: (A), human Stim1 transfected 293T: (B) and A-10 (C) whole cell lysates.| Application Notes | recommended for detection of Stim1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
