Application
Western blot analysis of NRAGE expression in non-transfected 293T: (A), mouse NRAGE transfected 293T: (B), Jurkat (C) and ALL-S1L (D) whole cell lysates.Application
Western blot analysis of NRAGE expression in mouse brain tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic staining of adnexal and epidermal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of NRAGE expression in non-transfected: (A) and human NRAGE transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Product Name | NRAGE Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NRAGE of human origin |
| Modification Notes | The neurotrophin family of growth factors (NGF) function to regulate neuronal differentiation, synaptic activity and neuronal survival, as well as axonal and dendritic growth. The melanoma-associated antigen (MAGE) family consists of a number of antigens recognized by cytotoxic T lymphocytes. Neurotrophin receptor-interacting MAGE homolog (NRAGE) binds the p75 neurotrophin re-ceptor and associates with the plasma membrane when NGF binds p75NTR. The critical factors for NRAGE association lie within the juxtamembrane domain of p75NTR. Overexpression of NRAGE stimulates cell cycle arrest and allows NGF-dependent apoptosis within sympathetic neuron precursors cells. NRAGE is expressed in the medulla oblongata during development and motor-neurons. Structural similarities suggest NRAGE, and the MAGE protein necdin, (Ndn), mediate cell cycle effects through a shared mechanism. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of NRAGE of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NRAGE of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NRAGE of human origin |
| Entrez Gene | 9500 |
|---|
Application
Western blot analysis of NRAGE expression in non-transfected 293T: (A), mouse NRAGE transfected 293T: (B), Jurkat (C) and ALL-S1L (D) whole cell lysates.
Application
Western blot analysis of NRAGE expression in mouse brain tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic staining of adnexal and epidermal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of NRAGE expression in non-transfected: (A) and human NRAGE transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Application Notes | recommended for detection of NRAGE of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
