Application
Western blot analysis of HILI2 expression in NTERA-2 cl.D1 whole cell lysate.| Product Name | HILI2 Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 471-560 mapping within an internal region of HILI2 of human origin |
| Modification Notes | HILI2 (Piwi-like protein 4) is a 852 amino acid protein encoded by the human gene PIWIL4. HILI2 belongs to the argonaute family and contains one PAZ domain and one Piwi domain. HILI2 is a cytoplasmic protein that is expressed in adult testis. It regulates spermatogenesis and primordial germ cell production and has an essential role in meiotic differentiation of spermatocytes and in self-renewal of spermatogonial stem cells. HILI2-null mice are of normal size and weight and have the expected life span. Homozygous HILI2-null females are fertile and have no obvious defects. However, HILI2-deficient males are infertile and show a meiotic progression defect in early prophase of meiosis I and progressive loss of germ cells with age. Mutant males show elevated expression of LINE-1 and intracisternal A particle (IAP) element transcripts in germ cell lineages. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HILI2 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 471-560 mapping within an internal region of HILI2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 471-560 mapping within an internal region of HILI2 of human origin |
| Entrez Gene | 143689 |
|---|
Application
Western blot analysis of HILI2 expression in NTERA-2 cl.D1 whole cell lysate.| Application Notes | recommended for detection of HILI2 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
