Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Immunofluorescence staining of normal mouse skin frozen section showing cytoplasmic staining in epithelial cells.Application
Western blot analysis of UKHC expression in Jurkat (A) and HeLa (B) whole cell lysates.| Product Name | UKHC Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 691-740 mapping within an internal region of UKHC of human origin |
| Modification Notes | The kinesin motor proteins include at least two forms of conventional kinesin encoded by different genes and designated as ubiquitous kinesin, which is expressed in all cells and tissues, or neuronal kinesin, which is expressed exclusively in neural cells. Kinesin is a microtubule associated protein comprised of three different structural domains. A considerable globular N-terminal domain regulates the hydrolysis of ATP and also microtubule binding while central coiled-coil domains promote heavy chain dimerization. Lastly, small globular C-terminal domains interact with kinesin light chains, membranous organelles and vesicles. Expression of ubiquitous kinesin heavy chain, also designated UKHC, is found subcellularly in areas of heavy vesicular trafficking such as the microtubule pathways of neural cells and also the Golgi of non-neural cell types. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ubiquitous kinesin heavy chain (UKHC) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 691-740 mapping within an internal region of UKHC of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 691-740 mapping within an internal region of UKHC of human origin |
| Entrez Gene | 3799 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Immunofluorescence staining of normal mouse skin frozen section showing cytoplasmic staining in epithelial cells.
Application
Western blot analysis of UKHC expression in Jurkat (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of ubiquitous kinesin heavy chain (UKHC) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
