Application
Western blot analysis of IRBP expression in non-transfected: (A) and mouse IRBP transfected: (B) 293T whole cell lysates.Application
Western blot analysis of IRBP expression in non-transfected: (A) and mouse IRBP transfected: (B) 293T whole cell lysates and mouse eye tissue extract (C).Application
Western blot analysis of IRBP expression in Y79 whole cell lysate.| Product Name | IRBP Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 948-1247 mapping at the C-terminus of IRBP of human origin |
| Modification Notes | Interphotoreceptor retinoid-binding protein (IRBP) is a retinoid and fatty acid binding protein expressed exclusively in the photoreceptor cells of the retina and pinealocytes of the pineal gland. The gene encoding human IRBP maps to chromosome 10q11.2. A putative trans-acting complex binds a cis-element in the IRBP promoter and fully activates transcription of the IRBP gene. Hypomethylation of cysteine residues in the IRBP promoters of bovine and murine photoreceptor cells permits expression of the IRBP gene. IRBP may be involved in shuttling retinoids between photoreceptors and the retinal pigment epithelium. IRBP exists as a glycoprotein with a four-fold repeat structure. Each 300 amino acid repeat contains one A and one B domain corresponding to the first 80 amino acids (A) and the other 220 amino acids (B). Along with S-antigen and opsin, the antigenic properties of IRBP induce posterior uveitis, which is characterized by the inflammation of the choroid and photoreceptor cell death. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of precursor and mature IRBP of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 948-1247 mapping at the C-terminus of IRBP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 948-1247 mapping at the C-terminus of IRBP of human origin |
| Entrez Gene | 5949 |
|---|
Application
Western blot analysis of IRBP expression in non-transfected: (A) and mouse IRBP transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of IRBP expression in non-transfected: (A) and mouse IRBP transfected: (B) 293T whole cell lysates and mouse eye tissue extract (C).
Application
Western blot analysis of IRBP expression in Y79 whole cell lysate.| Application Notes | recommended for detection of precursor and mature IRBP of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
