Application
Western blot analysis of hSNF2H expression in K-562 nuclear extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast cancer tissue showing nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of hSNF2H expression in non-transfected 293T: (A), human hSNF2H transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human ovary tumor showing nuclear localization.| Product Name | hSNF2H Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The SWI/SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2a) and Brg-1 (also designated SNF2b) are the ATPase subunits of the mammalian SWI/SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI/SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila Isw1, a BRM related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of hSNF2H of human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 753-1052 mapping at the C-terminus of hSNF2H of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 753-1052 mapping at the C-terminus of hSNF2H of human origin |
| Entrez Gene | 8467 |
|---|
Application
Western blot analysis of hSNF2H expression in K-562 nuclear extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast cancer tissue showing nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of hSNF2H expression in non-transfected 293T: (A), human hSNF2H transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human ovary tumor showing nuclear localization.| Application Notes | recommended for detection of hSNF2H of human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
