Application
Western blot analysis of SFXN5 expression in mouse brain tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | SFXN5 Antibody (N-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of SFXN5 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | The sideroflexin (SFXN) family is comprised of SFXN1, SFXN2, SFXN3, SFXN4 and SFXN5. SFXN1, also designated tricarboxylate carrier protein TCC, is the most highly characterized family member. The ubiquitously expressed SFXN1 protein resides as an integral protein of the mitochondrial inner membrane. It functions as an essential component of the shuttle system that transports mitochondrial acetyl-CoA into the cytosol, where lipogenesis occurs. The SFXN1 gene is mutated in flexed-tail (f/f) mice, which display axial skeletal abnormalities and a transient embryonic and neonatal anemia characterized by pathologic intramitochondiral iron deposits in erythrocytes. Therefore, SFXN1 is also thought to facilitate the transport of a component required for iron utilization into mitochondria. All SFXN family members show expression in pancreatic islet cells. SFXN5 displays a citrate transport activity and is primarily expressed in brain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SFXN5 of mouse and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SFXN5 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 94097 |
|---|
Application
Western blot analysis of SFXN5 expression in mouse brain tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
