Application
Western blot analysis of Rad51 expression in non-transfected: (A) and mouse Rad51 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in ductus seminiferus at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Rad51 expression in HeLa (A), K-562 (B), and A-431 (C) nuclear extracts.| Product Name | Rad51 Antibody (H-92) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-92 of Rad51 of human origin |
| Modification Notes | Rad52 family members (Rad50, Rad51B/C/D, Rad52, Rad54, MRE11) mediate DNA double-strand break repair (DSBR) for DNA damage that otherwise could cause cell death, mutation or neoplastic transformation. Rad51 (RECA, BRCC5) interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of Rad51. Rad52 forms a heptameric ring that binds single-stranded DNA ends and catalyzes DNA-DNA interaction necessary for the annealing of complementary strands. Rad52 can interact with Rad51. Rad54A of the DEAD-like helicase superfamily binds to double-strand DNA and induces a DNA topological change, which is thought to facilitate homologous DNA pairing and stimulate DNA recombination. Rad54B of the DEAD-like helicase superfamily binds to double-stranded DNA and displays ATPase activity in the presence of DNA. Rad54B is abundant in testis and spleen, and mutations of this gene occur in primary lymphoma and colon cancer. MRE11 (meiotic recombination 11, ATLD, HNGS1) is a nuclear 3'-5' exonuclease/endonuclease that associates with Rad50 and influences homologous recombination, telomere length maintenance, and DNA double-strand break repair. MRE11 is most abundant in proliferating tissues. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Rad51 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-92 of Rad51 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-92 of Rad51 of human origin |
| Entrez Gene | 5888 |
|---|
Application
Western blot analysis of Rad51 expression in non-transfected: (A) and mouse Rad51 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in ductus seminiferus at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Rad51 expression in HeLa (A), K-562 (B), and A-431 (C) nuclear extracts.| Application Notes | recommended for detection of Rad51 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
