Application
Western blot analysis of Telethonin expression in mouse skeletal muscle (A) and rat skeletal muscle (B) tissue extracts.Application
Immunofluorescence staining of methanol-fixed Sol8 cells showing cytoplasmic localization.| Product Name | Telethonin Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 58-167 of Telethonin of human origin |
| Modification Notes | Titin, also known as connectin, is a large protein involved in the temporal and spatial control of the assembly of the highly ordered sarcomeres (contractile units) of striated muscle (1). In addition to sarcomere assembly titin also functions to maintain the structural integrity of the contracting myofibrils within the muscle as well as organizing the machinery for condensation of chromosomes in dividing cells (1-3). Titin is a giant protein composed of 27,000 amino acids (1) and contains an autoregulated serine kinase catalytic domain as well as a calcium/calmodulin binding region that are involved in its activation (4,5). Activated titin phosphorylates the muscle protein telethonin, a sarcomeric protein abundant in heart and skeletal muscle, implicating titin activity in the reorganization of the cytoskeleton during myofibrillogenesis (4-6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Telethonin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 58-167 of Telethonin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 58-167 of Telethonin of human origin |
| Entrez Gene | 8557 |
|---|
Application
Western blot analysis of Telethonin expression in mouse skeletal muscle (A) and rat skeletal muscle (B) tissue extracts.
Application
Immunofluorescence staining of methanol-fixed Sol8 cells showing cytoplasmic localization.| Application Notes | recommended for detection of Telethonin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
