Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of lymphoid cells.Application
Western blot analysis of WIP expression in BJAB (A) and HL-60 (B) whole cell lysates.Application
Western blot analysis of WIP expression in non-transfected: (A) and human WIP transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining of follicle and non-follicle cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed BJAB cells showing cytoskeletal localization.Application
Western blot analysis of WIP expression in BJAB whole cell lysate.| Product Name | WIP Antibody (H-224) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 181-480 (deletion 314-389) mapping near the C-terminus of WIP of human origin |
| Modification Notes | Mutations in the Wiskott-Aldrich syndrome protein (WASP) often result in mmunodeficiency due to abnormal T cell and B cell activation (1,2). The 503 amino acid WAS-interacting protein (WIP) contains a number of domains implicated in actin-binding and several putative src homology-binding domains (3,4). The first 100 amino acids of WASP interact with amino acids 377-503 of WIP (3,4), and the majority of pathogenic mutations associated with WAS occur within the first 100 amino acids of WASP (5). The gene encoding human WIP maps to chromosome 2p23.3-q24.3 (4). Overexpression of WIP in the human B cell line BJAB increases F-actin content and cerebriform projections (4). While both WIP and Vav cooperate in the regulation of NF-AT/AP-1 gene transcription, the WIP-WASP complex is required for activation of NF-AT/AP-1 necessary for proper T cell function (6). A dysfunctional WIP-WASP complex may be implicated in the immunodeficient phenotype in WAS (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of WIP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 181-480 (deletion 314-389) mapping near the C-terminus of WIP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 181-480 (deletion 314-389) mapping near the C-terminus of WIP of human origin |
| Entrez Gene | 7456 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of lymphoid cells.
Application
Western blot analysis of WIP expression in BJAB (A) and HL-60 (B) whole cell lysates.
Application
Western blot analysis of WIP expression in non-transfected: (A) and human WIP transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining of follicle and non-follicle cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed BJAB cells showing cytoskeletal localization.
Application
Western blot analysis of WIP expression in BJAB whole cell lysate.| Application Notes | recommended for detection of WIP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
