Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of CBS expression in HeLa whole cell lysate.Application
Western blot analysis of CBS expression in non-transfected: (A) and human CBS transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CBS expression in non-transfected: (A) and mouse CBS transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CBS expression in non-transfected: (A) and human CBS transfected: (B) 293 whole cell lysates.Application
Western blot analysis of CBS expression in non-transfected: (A) and human CBS transfected: (B) 293 whole cell lysates.Application
Western blot analysis of CBS expression in rat pancreas (A), rat liver (B) and mouse pancreas (C) tissue extracts.| Product Name | CBS Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Strongly expressed in human liver and pancreas, with weaker expression in heart and brain, the cytoplasmic protein cystathionine b-synthase (CBS) operates in the first step of homocysteine transulfuration. CBS, which belongs to the cysteine synthase/cystathionine b-synthase family of proteins, catalyzes the formation of cystathionine from the thrombogenic amino acid homocysteine using pyridoxal phosphate cofactor. Allosteric activation by adenosyl-methionine regulates CBS activity. Deficiencies in CBS are assoc-iated with homocystinuria, a recessively inherited error in sulfur amino acid metabolism that affects many organs and tissues. Symptoms of homocytinuria include arteriosclerosis, thrombosis, dislocated optic lenses, mental retard-ation and skeletal abnormalities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CBS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 101-400 mapping within an internal region of CBS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 101-400 mapping within an internal region of CBS of human origin |
| Entrez Gene | 875 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of CBS expression in HeLa whole cell lysate.
Application
Western blot analysis of CBS expression in non-transfected: (A) and human CBS transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CBS expression in non-transfected: (A) and mouse CBS transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CBS expression in non-transfected: (A) and human CBS transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of CBS expression in non-transfected: (A) and human CBS transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of CBS expression in rat pancreas (A), rat liver (B) and mouse pancreas (C) tissue extracts.| Application Notes | recommended for detection of CBS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
