Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing apical membrane and cytoplasmic staining of glandular cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of IFITM1/2/3 expression in K-562 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic and membrane staining of lymphoid cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of IFITM1/2/3 expression in non-transfected 293: (A), human IFITM1 transfected 293: (B) and K-562 (C) whole cell lysates.| Product Name | IFITM1/2/3 Antibody (FL-125) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Interferons (IFNs) are potential antitumor agents, as they exhibit antiproliferative and differentiating properties, in addition to functioning in the defense against microbial infections. IFN exposure induces the regulation of expression levels of cellular proteins that mediate the pleiotropic effects of interferons. These effects may be mediated by soluble factors or by cell-cell interactions involving specific membrane proteins. The IFITM family of proteins are transmembrane proteins so named because their expression is IFN-inducible. IFITM proteins have been found upregulated in human colorectal carcinomas. Both mouse IFITM1 (also known as CD225) and IFITM3 demonstrate expression on the cell surfaces of primordial germ cells in a developmentally-regulated manner. They presumably modulate cell adhesion and influence cell differentiation. IFITM1 activity is required for primordial germ cell transit, and IFITM1 acts as a repulsive molecule by repelling non-IFITM1-expressing primordial germ cells from the mesoderm into the endoderm. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IFITM1, IFITM2, IFITM3 and to a lesser extent, other IFITM family members of human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-125 representing full length IFITM1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-125 representing full length IFITM1 of human origin |
| Entrez Gene | 8519 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing apical membrane and cytoplasmic staining of glandular cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of IFITM1/2/3 expression in K-562 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic and membrane staining of lymphoid cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of IFITM1/2/3 expression in non-transfected 293: (A), human IFITM1 transfected 293: (B) and K-562 (C) whole cell lysates.| Application Notes | recommended for detection of IFITM1, IFITM2, IFITM3 and to a lesser extent, other IFITM family members of human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
