Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing cytoplasmic and membrane staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed Sol8 cells showing cytoplasmic localization.Application
Western blot analysis of human recombinant UCP4 fusion protein.| Product Name | UCP4 Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | A significant portion of the metabolic rate of endotherm is attributable to counteracting uncoupling, wherein a flux of protons down the electrochemical gradient generates heat independently of ATP production. Uncoupling is apparent in thermogenic brown adipose tissue, which expresses tissue-specific uncoupling protein (UCP), suggesting that innate uncoupling and metabolic rate are regulated by UCPs. UCPs are a family of mitochondrial transporter proteins that are implicated in thermoregulatory heat production and maintenance of the basal metabolic rate. A brain-specific novel member of UCP family, UCP4, is most related to UCP3 and possesses features characteristic of mitochondrial transporter proteins. Unlike other known UCPs, UCP4 mRNAs are expressed in both fetal and adult brain tissues. Human UCP4, a 323 amino acid protein, has been speculated on its participation in apoptosis because of its early phylogenetic occurrence. Brain UCP4 mRNA rose by 1.5 fold in response to acute cold exposure, suggesting UCP4 is involved in tissue-specific thermoregulation and metabolic changes. The UCP-specific sequences are found in the first, second and fourth alpha-helices and are involved in fatty acid anion binding and translocation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of UCP4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 191-290 mapping near the C-terminus of UCP4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 191-290 mapping near the C-terminus of UCP4 of human origin |
| Entrez Gene | 9481 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing cytoplasmic and membrane staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed Sol8 cells showing cytoplasmic localization.
Application
Western blot analysis of human recombinant UCP4 fusion protein.| Application Notes | recommended for detection of UCP4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
