Application
Western blot analysis of Gl Syn expression in mouse liver tissue extract.Application
Western blot analysis of Gl Syn expression in non-transfected: (A) and human Gl Syn transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Gl Syn expression in non-transfected: (A) and mouse Gl Syn transfected: (B) 293T whole cell lysates.Application
Western blot analysis of glutamine synthetase expression in Hep G2 whole cell lysate.| Product Name | Gl Syn Antibody (FL-373) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Glutamine synthetase (GI Syn) forms a homooctamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Gl Syn of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-373 representing full length glutamine synthetase (Gl Syn) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-373 representing full length glutamine synthetase (Gl Syn) of human origin |
| Entrez Gene | 2752 |
|---|
Application
Western blot analysis of Gl Syn expression in mouse liver tissue extract.
Application
Western blot analysis of Gl Syn expression in non-transfected: (A) and human Gl Syn transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Gl Syn expression in non-transfected: (A) and mouse Gl Syn transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of glutamine synthetase expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of Gl Syn of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
