Application
Western blot analysis of Hemoglobin α expression in mouse heart tissue extract.Application
Western blot analysis of Hemoglobin α expression in K-562 whole cell lysate.Application
Western blot analysis of Hemoglobin α expression in human PBL whole cell lysate.| Product Name | Hemoglobin α Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Hemoglobin (Hgb) is coupled to four iron-binding, methene-linked tetrapyrrole rings (heme). The å (16p13.3; 5'-Ω-pseudoz-pseudo å2-pseudo å1-å2-å1-œ1-3') and ∫ (11p15.5) globin loci determine the basic hemoglobin structure. The globin portion of hemoglobin consists of two å chains and two ∫ chains arranged in pairs forming a tetramer. Each of the four globin chains covalently associates with a heme group. The bonds between å and ∫ chains are weaker than between similar globin chains, thereby forming a cleavage plane that is important for oxygen binding and release. High affinity for oxygen occurs upon relaxation of the å1-∫2 cleavage plane. When the two å1-∫2 interfaces are closely bound, hemoglobin has a low affinity for oxygen. Hb A, which contains two å chains plus two ∫ chains, comprises 97% of total circulating hemoglobin. The remaining 3% of total circulating hemoglobin is comprised of Hb A-2, which consists of two å chains plus two ∂ chains, and fetal hemoglobin (Hb F), which consists of two å chains together with two © chains. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection Hemoglobin &alpha of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 62-142 mapping at the C-terminus of Hemoglobin &alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 62-142 mapping at the C-terminus of Hemoglobin α of human origin |
| Entrez Gene | 3039 |
|---|
Application
Western blot analysis of Hemoglobin α expression in mouse heart tissue extract.
Application
Western blot analysis of Hemoglobin α expression in K-562 whole cell lysate.
Application
Western blot analysis of Hemoglobin α expression in human PBL whole cell lysate.| Application Notes | recommended for detection Hemoglobin &alpha of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
