Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic llocalization.Application
Western blot analysis of γ-GCSm regulatory subunit expression in A549 (A), MOLT-4 (B) and K-562 (C) whole cell lysates.| Product Name | γ-GCSm Antibody (FL-274) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Gamma-glutamylcysteine synthetase (©-GCS) is the rate limiting enzyme for glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. ©-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. The human gamma-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human ©-GCS gene encoding the regulatory subunit maps to chromosome 1p22-p21. The two subunits of ©-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of &gamma-GCSm of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-274 representing full length &gamma -GCSm of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-274 representing full length γ-GCSm of human origin |
| Entrez Gene | 2730 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic llocalization.
Application
Western blot analysis of γ-GCSm regulatory subunit expression in A549 (A), MOLT-4 (B) and K-562 (C) whole cell lysates.| Application Notes | recommended for detection of &gamma-GCSm of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
