Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of cytoglobin expression in non-transfected: (A) and human cytoglobin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of cytoglobin expression in non-transfected: (A) and mouse cytoglobin transfected: (B) 293T whole cell lysates.| Product Name | cytoglobin Antibody (FL-190) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Hemoglobin, myoglobin, neuroglobin, and cytoglobin belong to the globin family, porphyrin-containing proteins that function in oxygen transport and storage. Myoglobin contributes to intracellular oxygen storage and transcellular facilitated diffusion of oxygen in skeletal and cardiac muscle. Neuroglobin is an oxidative stress-responsive sensor for signal transduction in the brain. Hemoglobin contributes to oxygen storage and diffusion of oxygen in blood tissue. Cytoglobin (also designated histoglobin), is a ubiquitous protein that facilitates diffusion of oxygen through tissues and acts as a scavenger for nitric oxide or other reactive oxygen species. It binds O(2) via its heme and also has a protective function during oxidative stress. Cytoglobin, a hexacoordinate hemoglobin, shares less than 30% identity with other human hemoglobins and is widely expressed in a wide array of tissues including fibroblasts and nerve cell populations. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of cytoglobin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-190 representing full length cytoglobin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-190 representing full length cytoglobin of human origin |
| Entrez Gene | 114757 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of cytoglobin expression in non-transfected: (A) and human cytoglobin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of cytoglobin expression in non-transfected: (A) and mouse cytoglobin transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of cytoglobin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
